Modified automated determination of 2,3-diphosphoglycerate in whole blood.
نویسنده
چکیده
A modification is described of the automated determination of 2,3-diphosphoglycerate (DPG) in blood [Grisolia, S., et al.,Anal. Biochem. 31, 235 (1969)J. Modifications in the manifold result in a sensitive, noise-free, rapid system and the modifications in the preparations of the reagents ensure stability of the diluted standards and blood samples for at least three weeks. Samples are run at the rateof 60/h and sample size can be as small as 5 l of whole blood. The coefficientof variation of the overall determination of automated DPG and manual hemoglobin is 3.6% and the SD is ±0.77 moI/g Hb. The normal range is 14.6 ± 2.2(SD) mol/g hemoglobin.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 18 9 شماره
صفحات -
تاریخ انتشار 1972